Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Perkin Elmer US LLC Capillary Injector with PPC, 120 V
The AutoSystem XL must be PPC (Programmable Pneumatic Control) ready. If not, a PPC upgrade kit (N6120146) is required. Installation by PerkinElmer Service is required, but not included.
Perkin Elmer US LLC POC Injector with PPC Add-On Kit, 240 V
Part of the Programmed-Temperature On-Column Injector System. The AutoSystem XL or Clarus GC must be PPC (Programmable Pneumatic Control) ready. If not, a PPC upgrade kit (N6120146) is required. Installation by PerkinElmer Service is required, but not included.
Molecular Devices Fluorescence Polarization Filters, 590 nm, 20 nm
Fluorescence polarization emission filter set for fluorescence polarization and related fluorescence detection assays. The set includes two polarized emission filters and a holder for mounting in compatible filter-based fluorescence instruments.
- Set of two polarized emission filters for fluorescence polarization measurements.
- Center wavelength 590 nm, bandwidth 20 nm.
- One vertical and one horizontal polarized filter for polarization assays.
- Includes holder for secure filter mounting.
- Designed for use in fluorescence polarization and related fluorescence detection workflows.
Revvity Health Sciences Inc HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-FGFR1 (Tyr653/654) Detection Kit, 10,000 Assay Points
Molecular Devices Time-Resolved Filter, 350 nm, 60 nm Bandwidth
This time-resolved excitation filter selects short-wavelength excitation light centered at 350 nm with a 60 nm full width, for use in time-resolved fluorescence (TRF) assays on compatible filter-based microplate readers. It is intended for applications that require narrow-band excitation around 350 nm for TRF measurements.
- Time-resolved excitation filter.
- Center wavelength 350 nm.
- Bandwidth 60 nm (full width).
- Optimized for time-resolved fluorescence assays.
- Compatible with SpectraMax iD5 filter module and similar readers.
- Fits standard filter holder assemblies in compatible readers.
Revvity Health Sciences Inc HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK2 Detection Kit, 50,000 Assay Points
HTRF Human Total JAK2 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATM Detection Kit, Control Lysate
HTRF Human Total ATM Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays
Revvity Health Sciences Inc HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points
HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points