Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit Standard, 3 Vials
HTRF Human IL-1β High Performance Detection Kit Standard, 3 Vials
Revvity Health Sciences Inc HTRF Human & Mouse Total RBM39 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total RBM39 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL12/23 p40 Detection Kit, Standard, 3 Vials
HTRF Human IL12/23 p40 Detection Kit, Standard, 3 Vials
Revvity Health Sciences Inc HTRF Human Total CDK1 Detection Kit, 50,000 Assay Points
HTRF Human Total CDK1 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total GSPT1 Detection Kit, Control Lysate
HTRF Human Total GSPT1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human CCL22 Detection kit, 10,000 Assay Points
HTRF Human CCL22 Detection kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-2 High Performance Detection Kit Standard, 3 Vials
HTRF Human IL-2 High Performance Detection Kit Standard, 3 Vials
Revvity Health Sciences Inc HTRF MDM2 Binding Kit, 100,000 Assay Points
HTRF MDM2 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Mouse Phospho-STING (Ser365) Detection Kit, 50,000 Assay Points
HTRF Mouse Phospho-STING (Ser365) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total CDK1 Detection Kit, 500 Assay Points
HTRF Human Total CDK1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-RAB10 (Thr73) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-RAB10 (Thr73) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Total Myd88 Detection Kit, 10,000 Assay Points
HTRF Total Myd88 Detection Kit, 10,000 Assay Points
Molecular Devices Fluorescence Excitation Filter, 370 nm, Time-Resolved, Single Unit
Molecular Devices 370 nm time-resolved fluorescence filter provides excitation at 370 nm for time-resolved fluorescence assays. It is supplied as a single replacement optical filter for compatible fluorescence instruments.
- Provides excitation centered at 370 nm.
- Designed for time-resolved fluorescence measurements.
- Supplied as a single replacement optical filter.
- Intended for use in compatible fluorescence detection instruments.
- Helps improve excitation specificity for time-resolved assays.
Molecular Devices Absorbance Filter, 640 nm, 8 nm BW
The AD 340 absorbance photometric filter provides a narrowband 640 nm passband for absorbance measurements in filter-wheel microplate readers. It is supplied as a single replacement filter for compatible readers and supports assays that require red-wavelength detection.
- Absorbance detection at 640 nm center wavelength.
- Bandwidth 8 nm.
- Designed for filter-wheel microplate reader configurations.
- Single replacement filter for maintenance and assay reproducibility.
- Supports red-wavelength absorbance assays and endpoint measurements.